Qiagen tissuelyser ii pdf download

Supplemental material and methods specimen collection and. Disruption and homogenization using the tissuelyser ii gives yields comparable. Aug 28, 2018 genomic dna gdna extracted from fresh, freezedried, and silica geldried plant tissue. Manuals bioinformatics software and services qiagen. Jul 07, 2017 qiagen tissuelyser ii sample disruption 11843. Qiacube ht laboratory equipment pdf manual download. Qiaex ii handbook qiaex ii agarose gel extraction protocol. The purified dna is up to 50 kb in size, with fragments of 20 30 kb predominating. Determine the amount of tissue and place it into the qiazol lysis reagent. Extraction buffer exb plus provided in the qproteome ffpe kit qiagen cat. Equipnet is constantly receiving used qiagen equipment through.

Bioinformatics software and services qiagen digital insights. For highly specific and sensitive multiplex pcr without optimization requirements. Rnalater rna stabilization reagent qiazol lysis reagent. Use of these in next generation sequencing ngs is a priority. Wo20161892a1 composition and method for disrupting tissue. Tissuelyser adapter set 2 x 96 from qiagen selectscience. Qiagen allprep kits are designed to isolate dna and total rna from small quantities of starting material. The tissuelyser ii is an integral part of qiagen s complete solution for sample management from sample collection to purification and analysis of dna, rna, and protein. Ursodeoxycholic acid enriches intestinal bile salt hydrolase. Keep the insert of the tissuelyser lt adapter at room temperature. Tissuelyser ii tissuelyser ii delivers medium to highthroughput disruption of up to 2 x 96 samples of tissue, plant material, yeast or bacteria in less than 5 minutes. Qiagen offers adapter sets for 2 x 96 collection microtubes 1.

The remaining steps were carried out according to the manufacturers recommendations, and the dna eluted in 100 ul elution buffer. The purchaser must determine the suitability of the product for its particular use. When using the qiagen tissuelyser lt or tissuelyser ii, process the sample according to the protocol cation of rna or multiple analytes frompurifi animal and human tissues in the tissuelyser handbook. Apr 28, 2017 tissuelyser lt tissuelyser lt is a small bead mill that enables low to mediumthroughput sample disruption, of up to 12 samples at the same time. This length of dna readily amplifies with high efficiency and is ready for use in standard downstream applications such as sequencing. Tissuelyser ii the tissuelyser ii provides highthroughput processing for simultaneous, rapid, and effective disruption of up to 192 biological samples, including all types of animal tissue. Jun 02, 2014 add the appropriate volume of buffer rlt see table 8, and homogenize by passing the lysate at least 5 times through a blunt 20gauge needle fitted to an rnasefree syringe. Quality assessment included total yield, percent dsdna. Qiagen retsch tissuelyser ii bead mill for sale 591 used retsch grindomix gm 200 knife mill sale advatec low friction thermal barrier coatings based on. All instructions and protocols in this handbook apply to both the tissuelyser ii and the.

Qiagen offers advanced and innovative solutions for all of your assay and sample equipment needs. The kit uses qiagens secondgeneration inhibitor removal technology irt, and is intended for use with. Servicing of the tissuelyser ii should only be performed by qiagen or an authorized agent. Qiagen clinical insight bioinformatics software and.

Do not continue to use the tissuelyser ii or tissuelyser accessory if it is damaged. Extraction was optimised with a 10 min 70 c incubation quigley et al. Tissuelyser lt adapter, 12tube from qiagen selectscience. Standard operating procedure sop for dnarna extraction. See the tissuelyser lt handbook or the tissuelyser handbook. I am sure you can buy equivalent beads at vwr or fisher. Laboratory equipment qiagen tissuelyser ii user manual 48 pages. The tissuelyser lt is a small bead mill which provides fast, effective disruption of up to 12 samples at the same time. Sample disruption tissuelyser ii for medium to highthroughput sample disruption for molecular analysis 85300 tissuelyser lt for low to mediumthroughput sample disruption for molecular analysis 85600 powerlyzer 24 homogenizer 110220 v for the most efficient and complete lysis and. Safety information 6 tissuelyser ii user manual 042010 with accessories approved by qiagen see section 3. Evaluating the impact of dna extraction method on the. Rna extraction from plants using the rneasy plant mini kit.

Lt is comparable to that achieved with the wellestablished tissuelyser ii figures 6 and 810. Sample to insight beadbasedhomogenization for unbiased nucleic acid representation metal 2. Qiagen clinical insight qci is an evidencebased decision support software intended as an aid in the interpretation of variants observed in genomic sequencing data. Place the tubes into the insert of the tissuelyser lt adapter, and incubate at room temperature for 2 min to avoid freezing of lysis buffer in step 3. Various rat tissues 25 mg each stabilized in allprotect tissue reagent were disrupted using the tissuelyser lt or tissuelyser ii. For disruption of up to 48 or 192 biological samples 48 pages. Processing of up to 2 x 96 samples takes as little as 2 5 minutes. Excise the tissue sample from the animal or remove it from storage. Qiagen qiaamp kits provide a fast, easy, and reliable method to purify total dna from small quantities of human tissue or blood. The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads.

Qiagen clc main workbench is used by tens of thousands of researchers all over the world for dna, rna and protein sequence data analysis. These are available online in convenient and compact pdf format at. Disruption and homogenization using the tissuelyser lt. Does anyone use the tissuelyser ii qiagen equipment to.

Qiagen clc genomics workbench is a powerful solution that works for everyone, no matter the workflow. The purified dna and rna are ready for use in standard downstream applications such as dna amplification and expression. Following incubation at 65 c and chloroform extraction, the crude dna extract was digested for 30 min at 37 c with rnase a 20 lg prior to isopropanol precipitation. We stiil use pestle and mortar which is very timeconsuming and we would like to speed. Our inventory of used qiagen equipment includes various detectors, dna synthesizers, sample equipment, liquid handlers, and much more. Do not continue to use the tissuelyser ii or tissuelyser. Cuttingedge technology, unique features and algorithms widely used by scientific leaders in industry and academia make it easy to overcome challenges associated with data analysis. Should any product fail to perform satisfactorily due to any reason other. Laboratory equipment qiagen tissuelyser ii user manual. The powerfood microbial dna isolation kit was then used as per the manufacturers instructions.

For each method, an extraction blank pcrgrade water was used to ascertain potential kit andor reagent contamination. Does anyone have experience with tissue lyser from qiagen. Alternatively, a grinding jar set can be used to process large samples. Tissuelyser 3 mm bead dispenser, 96well from qiagen. It has been stored under unfavorable conditions for a prolonged period. Tissuelyser handbook university of texas at arlington. Advanced analysis, workflow and interpretation software accessing genomic and clinical knowledge from over 20 million references. If the tissuelyser ii is not in use, switch it off at the power switch.

Qiagen focuses on breaching great success and development in their company to better serve their consumers. Samples were processed on a tissuelyser ii qiagen, venlo, netherlands for 10 min. Aug 24, 2018 a detailed investigation of the porcine skin and nose microbiome using universal and staphylococcus. For disruption of up to 48 or 192 biological samples. Add the appropriate volume of buffer rlt see table 8, and homogenize by passing the lysate at least 5 times through a blunt 20gauge needle fitted to an rnasefree syringe. The tissuelyser adapter set 2 x 24 contains a pair of tl reactiontube holders, 24tube. For efficient disruption of up to 48 or 192 samples in parallel on the tissuelyser ii. Composition and method for disrupting tissue material download pdf. Comparison of dna extraction methods for human gut. Qiagen tissuelyser ii at 30 fs hz three times for 30 seconds, with placement of the samples on ice in between bead beating steps. Comparative evaluation of dna extraction methods from feces. Samples of gdna extracted from a clover leaf tissue or grass pseudostem b rice pseudostem and c arabidopsis thaliana leaf tissue using the highthroughput protocol were resolved and visualised by electrophoresis in an agarose lithium borate buffer 0. Its wide variety of features are presented in an intuitive graphical userinterface, for which advanced computer skills are not required.

Tissuelyser ii user manual 052016 7 it or the line power cord appears to be damaged. Qiagility laboratory equipment pdf manual download. If using the tissuelyser lt or tissuelyser ii, add the stainless steel beads of choice 1 x 5 mm or 2 x 7mm to the tube before the addition of qiazol. Purification of total rna, including small rnas, from human leukocytes 38 appendix e.

The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic. Qiagen clc genomics workbench qiagen digital insights. Human faecal aliquots 200 mg and murine caecal content were lysed using the qiagen tissuelyser ii bead beater 25 hz for 20 minutes, with dna extracted using the. The initial step in the performance of the assay was the performance of realtime pcr, using the lightcycler 480 roche diagnostics. View and download qiagen tissuelyser ii user manual online.

Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for. Carbonyl reduction of nnk by recombinant human lung enzymes. Qiagen multiplex pcr kit, x 50l rxns, genomic dna and cdna sample, pcr amplification reaction, 5 3 exonuclease enzyme activity, easytouse and costeffective, for highly specific and sensitive multiplex pcr without optimization requirements, includes 2x qiagen. This small bead mill has high throughput with highspeed shaking for the disruption or homogenization of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. Use of ffpederived dna in next generation sequencing. This german made shaker appears in good condition without cracks in the plastic cover or other damage to the housing or workings.

Usually after dna purification, 260280 ratio will ranging between 1,8 2 pure dna but all of my purification result shows 260280 ratio higher than 2 between 2 2,5. The realtime pcr modules transform threshold cycle c t values to calculated results for gene and mirna expression, somatic mutation detection and copy number measurements. This throughput matches that of the qiacube, which automates sample preparation using trusted qiagen spincolumn kits. Cranberry microsatellite marker development from assembled. For disruption of up to 48 or 192 biological samples 48 pages laboratory equipment qiagen ez1 advanced xl user manual 100 pages. For disruption of up to 48 or 192 biological samples 48. The tissuelyser lt is compact so you can keep it nearby for easy access. Qiagen provides stainless steel beads and tungsten carbide beads for use in sample disruption with tissuelyser systems. Highspeed shaking of sample material and a single bead in a collection microtube or 2 ml microcentrifuge tube for a few minutes allows rapid and efficient disruption of the sample. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation in cell culture. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation, such as the qiasymphony sp. Tissueruptor tissuelyser lt tissuelyser ii throughput samplesrun 1 up to 12 up to 48 or 192 disruption technology rotorstator bead mill bead mill disruption time 30 seconds 40 seconds to 5 minutes 20 seconds to 5 minutes table 1. Simultaneous disruption and homogenization is achieved through highspeed shaking of samples in 2 ml. Lung 60 50 40 30 20 10 0 tissuelyser lt tissuelyser ii liver heart brain skin 400 300 200 100 0 heart brainlung tissuelyser lt tissuelyser ii figure 6.

Used qiagen mm300 tissuelyser shaker mixer grinder agitator. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. Archival tissues represent a rich resource for clinical genomic studies, particularly when coupled with comprehensive medical records. Qiagen disruption systems for a range of throughputs. For mouse samples, one fecal pellet was placed into a 2 ml sterile roundbottom tube containing 500. Tissuelyser adapter set 2 x 24 from qiagen selectscience. The software evaluates genomic variants in the context of published biomedical literature, professional association guidelines, publicly available databases and annotations, drug. Preparation of cytosolic and microsomal fractions was performed as described previously. Nine formalinfixed paraffinembedded ffpe dna extraction methods were evaluated using twelve ffpe samples of varying tissue types. Generates a prerun report to assist with workspace setup for the current run. Standard operating procedure sop for dna extraction with. All microsomes were washed once with trisbuffered saline ph 7.

Transfer up to 30mg fresh or frozen tissue to the precooled tubes and incubate for another 15 min on dry ice. These are available online in convenient and compact pdf. Qiagen tissuelyser ii sample disruption 11843 youtube. Checkout popular qiagen categories laboratory equipment manuals. Qiagen is the leading provider of innovative sample and assay technologies, enabling the isolation and. Qiagen clc genome finishing module is an addon to qiagen clc genomics workbench, designed to accelerate and simplify genome finishing, and make this process accessible to life scientist without deep understanding of bioinformatics. We used 2030mg of tissue material in 600ll of buffer rlt plus with 6ll bmercaptoethanol. Safety data sheets sds for any qiagen or preanalytix product can be downloaded from. Subsequently, the samples were heated at 95c for 15 minutes. For sections from pfpe page 16 tissue, we recommend operating the tissuelyser lt or tissuelyser ii for 2 min at 25 hz. It has been subjected to severe transport stresses. Place a qiaquick spin column in a provided 2 ml collection tube or into a vacuum manifold. Various rat tissues were disrupted using the tissuelyser lt or.

Real time access and analysis of over 40 genomic and clinical databases covering over 33,000 diseases. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation. Add 1 gel volume isopropanol to the sample and mix. There are minor cosmetic scratches on the top plate, but nothing serious. It ramps up to 30 shakes per second and the timer works as it should. Ive read many protocols, help guides and specifically the troubleshooting guide in the kit protocol, but the number one piece of advice im seeing is to use the tissuelyser andor tissueruptor. This easytouse instrument automates purification of dna, rna, and protein from 196 samples. I asked precellys similar question and they suggested 2 3 cycles 40 second each with ck28 beads ceramic 2.

Disruption and homogenization using the tissuelyser lt or tissuelyser ii. The qiagen tissuelyser lt sample disruptor provides quick and efficient disruption in up to 12 samples. Storage, quantification, and determination of quality of rna 41 ordering information 44. The samples were subjected twice to bead beating using the qiagen tissuelyser ii at a frequency of 30 hz for 1 min each with a 5 min rest on ice in between, and then centrifuged. Qiagen clc main workbench bioinformatics software and. The tissuelyser enables fast and uniform disruption of animal and human tissues, plant tissues, bacteria, and yeast in various sample volumes in several formats. View and download qiagen qiagility user manual online. The lysate generated from this step was then prepared using the high pure pcr template preparation kit. Tissue homogenization was performed with a qiagen tissuelyser ii in 2 ml tubes with a 5mm bead at 22 hz for 2 min. For highthroughput disruption of biological samples pdf 89kb. Mechanical cell rupture was performed using a qiagen tissuelyser ii at 30 hz for 2 x 50 s, as applicable. The ngs module supports the analysis of qiagen s qiaseq ngs library kits and target enrichment panels.

Equipnet is constantly receiving used qiagen equipment through our exclusive contracts with our clients. In addition, they provide a fast and simple method for the preparation of dna and column purified rna from human tissues. May 2016 tissuelyser ii user manual gtac home page. Tissuelyser ii laboratory equipment pdf manual download. Sequencebased analysis of the bacterial and fungal. The system provides the same highquality results as other trusted qiagen purification technologies. The geneglobe data analysis center is a complimentary resource for analyzing realtime pcr or ngs data.